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Note for Guidance for Food Contact Materials
Page 106 of 126
Note 3:
Two primary solutions of analyte standard should be
prepared and checked against one another with one dilution
only. If there is agreement within 5% then further dilute
standards are made from only one of the primary standards.
Note 4:
Avoid weights of analyte and internal standard larger than
150 mg and also avoid volumes of solvent greater than
100 ml.

(CONDITIONS) =
set out conditions of storage and maximum storage time for
solutions, as obtained from stability tests.

4. APPARATUS:

4.1 [SPECIAL] =
set out special equipment e.g.:

-
gas chromatograph, equipped with:
automatic headspace sampler
alkali
flame-ionisation
detector
- chromatographic
column
- etc.
Note 5:
set out column requirements, such as:
-
the column must exhibit reasonable peak shape with
respect to half-width and asymmetry and must
permit the separation of analyte and internal standard

-
the column must exhibit minimum overlap of peaks
of analyte and internal standard and other
substances. A check should be specifically carried
out on interference with the internal standard.
- etc.
Note 6:
set out examples of columns that have been found suitable
for analyte determination - include details of type,
dimensions, column flow, temperature etc.

5. SAMPLES:
(CONDITIONS) =
set out conditions of storage of samples

Note 7:
Analytical determinations should be carried out on duplicate
samples, these being duplicate portions of [MATRIX], with
at least duplicate measurements (injections) of the final
extract.

5.1 Test
sample
preparation:
(DESCRIPTION) =
set out test sample preparation.

5.2 Calibration
sample
preparation: