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REF_N
CAS_N
NAME
SYNONIM
DIR
RESTRICTIONS
AND
SPECIFICATIONS
SCF
List
EFSA/SCF_OP
PL
CT
72
mutagenicity studies, negative; on the
impurity crotonic acid, two mutagenicity
tests, negative.
(CS/PM/1510 of 25.03.92; CS/PM/2317 and
2538 of 10.02.95).
Toxicologically acceptable provided the
following specifications are met:

STRUCTURAL FORMULA
CH3

CH3 O CH2 O

[-0-CH-CH2-C-]m-[O-CH-CH2-C-]n

n/(m + n) greater than 0 and less or equal to
0.25


DESCRIPTION
These copolymers are produced by the
controlled fermentation of Alcaligenes
eutrophus using mixtures of glucose and
propanoic acid as carbon sources. The
organism used has not been genetically
engineered and has been derived from a
single wild-type organism Alcaligenes
eutrophus strain H16 NCIMB 10442.
Master stocks of the organism are stored as
freeze-dried ampoules. A
submaster/working stock is prepared from
the master stock and stored in liquid
nitrogen and used to prepare inocula for the
fermenter. Fermenter samples will be
examined daily both microscopically and for
any changes in colonial morphology on a
variety of agars at different temperatures.
The copolymers are isolated from heat
treated bacteria by controlled digestion of
the other cellular components, washing and